Journal: Biophysical Journal
Article Title: Varying the position of phospholipid acyl chain unsaturation modulates hopanoid and sterol ordering
doi: 10.1016/j.bpj.2024.06.002
Figure Lengend Snippet: PC isomers interact differently with Chol and Dpop. While Chol ordering of PC increases as the double bond position is shifted further from the headgroup, Dpop only exhibits an ordering effect with Δ11-PC. ( a ) Chemical structure of di-unsaturated PC isomers, Chol, and Dpop and ( b ) isotherms of the corresponding lipids at 20°C, ∗∗∗ (F = 12.4, p < 0.0005). ( c ) Isotherms of PC isomers mixed with Chol (2:1) at 20°C. ∗∗ (F = 7.75, p < 0.005) MANOVA. ( f ) Isotherms of PC mixture with Dpop (2:1) at 20°C. ∗∗ (F = 8.18, p < 0.005) MANOVA. ( d and g ) Energy of interaction (ΔGmix) of lipid pairs with Chol and Dpop, respectively, during compression. Error bar represents standard deviation. n.s. p > 0.5 and ∗ p < 0.05, unpaired t -test. ( e and h ) Condensing effect of Chol and Dpop on PC isomers calculated at 30 mN/m. Error bar represents standard deviation. n.s. p > 0.5, ∗ p < 0.05, and ∗∗ p < 0.005, unpaired t -test. To see this figure in color, go online.
Article Snippet: Isotherms were recorded using a 70 cm 2 Teflon Langmuir trough fitted with a motorized compression barrier equipped with a pressure sensor (Kibron DeltaPi).
Techniques: Standard Deviation